| 000 | 03750nam a22005415i 4500 | ||
|---|---|---|---|
| 999 |
_c2246 _d2246 |
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| 001 | 978-3-319-16259-1 | ||
| 003 | DE-He213 | ||
| 005 | 20200804163747.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 150508s2015 gw | s |||| 0|eng d | ||
| 020 | _a9783319162591 (ebook) | ||
| 020 | _a9783319162584 | ||
| 024 | 7 |
_a10.1007/978-3-319-16259-1 _2doi |
|
| 040 |
_aSpringer Nature Metadata _beng _cMIU _dMIU_ain |
||
| 050 | 4 |
_aS494.5 _b.T55 2015 |
|
| 072 | 7 |
_aPSTL _2bicssc |
|
| 072 | 7 |
_aSCI011000 _2bisacsh |
|
| 072 | 7 |
_aPST _2thema |
|
| 072 | 7 |
_aTCB _2thema |
|
| 100 | 1 |
_aTill, Bradley J. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut |
|
| 245 | 1 | 0 |
_aLow-Cost Methods for Molecular Characterization of Mutant Plants : _bTissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / _cby Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger. |
| 250 | _a1st ed. 2015. | ||
| 264 | 1 |
_aCham : _bSpringer International Publishing : _bImprint: Springer, _c2015. |
|
| 300 |
_aX, 35 p. 9 illus., 3 illus. in color. _bonline resource. |
||
| 505 | 0 | _aIntroduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. . | |
| 506 | 0 | _aOpen Access | |
| 520 | _aThis book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits. | ||
| 650 | 0 | _aPlant breeding. | |
| 650 | 0 | _aBiology—Technique. | |
| 650 | 0 | _aNucleic acids. | |
| 650 | 1 | 4 |
_aPlant Breeding/Biotechnology. _0https://scigraph.springernature.com/ontologies/product-market-codes/L24060 |
| 650 | 2 | 4 |
_aBiological Techniques. _0https://scigraph.springernature.com/ontologies/product-market-codes/L28000 |
| 650 | 2 | 4 |
_aNucleic Acid Chemistry. _0https://scigraph.springernature.com/ontologies/product-market-codes/L14011 |
| 700 | 1 |
_aJankowicz-Cieslak, Joanna. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut |
|
| 700 | 1 |
_aHuynh, Owen A. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut |
|
| 700 | 1 |
_aBeshir, Mayada M. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut |
|
| 700 | 1 |
_aLaport, Robert G. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut |
|
| 700 | 1 |
_aHofinger, Bernhard J. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut |
|
| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9783319162607 |
| 776 | 0 | 8 |
_iPrinted edition: _z9783319162584 |
| 776 | 0 | 8 |
_iPrinted edition: _z9783319386676 |
| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-3-319-16259-1 |
| 942 |
_2lcc _cBK |
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