Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger.
By: Till, Bradley J [author.]
Contributor(s): Jankowicz-Cieslak, Joanna [author.] | Huynh, Owen A [author.] | Beshir, Mayada M [author.] | Laport, Robert G [author.] | Hofinger, Bernhard J [author.] | SpringerLink (Online service)
Publisher: Cham : Springer International Publishing : Imprint: Springer, 2015Edition: 1st ed. 2015Description: X, 35 p. 9 illus., 3 illus. in color. online resourceISBN: 9783319162591 (ebook); 9783319162584Subject(s): Plant breeding | Biology—Technique | Nucleic acids | Plant Breeding/Biotechnology | Biological Techniques | Nucleic Acid ChemistryAdditional physical formats: Printed edition:: No title; Printed edition:: No title; Printed edition:: No titleLOC classification: S494.5 | .T55 2015Online resources: Click here to access onlineItem type | Current location | Collection | Call number | Status | Notes | Date due | Barcode |
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Book | MILA University Central Library Red Spot | Reference | S494.5 .T55 2015 (Browse shelf) | Available | School of Science and Engineering | 0003125 |
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Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. .
Open Access
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
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